1. Place the microscope about an inch from the edge of the work table.
2. Position the LPO above the center of the stage. Adjust the iris diaphragm to its biggest opening. If there is an Abbe condenser just below the center of the stage, move it until it is in its uppermost position.
3. Adjust the mirror to get the right illumination.
4. Place the slide with specimen beneath the LPO. Once the specimen is focused, switch the LPO to HPO.

To keep the microscope in good working condition, make sure to handle and use it with care. In handling the instrument, hold it by its arm with one hand while your other hand is under its base. Before and after using it, wipe it clean using a piece of tissue paper or muslin cloth. Use lens paper to clean its lenses.